Twophoton laser scanning fluorescence microscopy and its applications 雙光子激光掃描熒光顯微鏡及其應用
Fluorescence microscopy a form of microscopy in which fluorescent probes are added to the material being investigated 熒光顯微術:用來觀察研究插入熒光探針后的物體的一種顯微技術。
Mycobacteria can also be stained with auramine and viewed with fluorescence microscopy , in which acid fast bacilli now appear as glowing yellow rods 分枝桿菌也能被金胺染色,熒光顯微鏡下抗酸桿菌為發(fā)黃*色熒光的桿菌。
Biofilm containing srb was observed on the surface of 316l ss by fluorescence microscopy in medicated postgate ' s c medium inoculated srb 熒光顯微鏡觀察發(fā)現(xiàn),在接種srb的修正的postgate ' sc培養(yǎng)基中, srb在316lss表面附著發(fā)展并形成生物膜。
After 12h - long transport , 6 ( 5 ) cf could be detected in the stock by using fluorescence microscopy . the result of transport was accord with the histological observation 證實該時期6 ( 5 ) cf可以自接穗跨過嫁接面向砧木運輸,這與組織學觀察結果一致。
For the first time , we observed the transformation of the j - aggregates morphologies depending on the surrounding humidity by fluorescence microscopy and atomic force microscopy 利用熒光顯微鏡和原子力顯微鏡我們第一次觀察到j -聚集體的形貌隨著濕度的變化而變化。
Through many years " hard work , we successfully developed the first commercial laser confocal scanning fluorescence microscopy and the first gene - chip scanner in our country 通過多年的努力奮斗,成功地開發(fā)出國內首臺商品化的激光共聚焦掃描熒光顯微鏡,并且成功開發(fā)出國內首臺基因芯片掃描儀。
In order to get the soluble recombinant eo protein and inspect the protein expression status convinently , the egfp and eo gene were ligated into baculovirus transfer vector . with the co - transfecting sf9 cells of baculovirus recombinant transfer vector and linearized viral dna , and plaque purification in the posttransfection procedure , the pure recombinant baculovirus were harvested , which infected the sf9 cells for amplifying to generate a p - l stock . . in the meantime , the fluorescence microscopy detection indicated expressed egfp protein to confirm the heterogenous protein expression of recombinant baculovirus . the pi - stock from a pure plaque was used to generate a high liter p - 2 stock , which was determined in liter as 1 . 14 107pfu / ml by performing a plaque assay . when a volume of p - 2 stock infected the sf9 cells with moi 5 - 10 for expression , the strong fluorescence was obeserved on the day 3 of postinfection 此外,為了得到可溶性重組eo蛋白并便于觀察重組蛋白的表達情況,我們將egfp基因與eo基因相連插入昆蟲桿狀病毒轉移載體中,與線性桿狀病毒dna共轉染sf9細胞后通過噬斑純化得到純的重組桿狀病毒,將其感染sf9細胞制備p1種子液,同時用熒光顯微鏡觀察綠色熒光蛋白的表達情況剔除表達效果差的重組桿狀病毒。再用p1種子液感染sf9細胞制備高效價的p2種子液。通過病毒液的梯度稀釋和噬斑測定,確定p2種子液的病毒滴度達1 . 14 10 ~ 7pfu ml 。
英文解釋
light microscopy in which the specimen is irradiated at wavelengths that excite fluorochromes